Impact of fungal hyphae on growth and dispersal of obligate anaerobic bacteria in aerated habitats
Impact of fungal hyphae on growth and dispersal of obligate anaerobic bacteria in aerated habitats
Abstract Anoxic microsites arising in fungal biofilms may foster the presence of obligate anaerobes even in well-areated environments. Here, we analyzed whether and to which degree fractal hyphae of Coprinopsis cinerea thriving in oxic habitats enable the germination, growth, and dispersal of obligate anaerobic soil bacterium Clostridium acetobutylicum. Time-resolved optical oxygen mapping, microscopy and metabolite analysis revealed the formation and persistence of anoxic circum hyphal niches allowing for spore germination, growth and fermentative activity of the obligate anaerobe in an otherwise oxic environment. Hypoxic liquid films containing 80 ± 10% of atmospheric oxygen saturation around single air-exposed hyphae thereby allowed for efficient clostridial dispersal amid spatially separated (>0.5 cm) anoxic sites. Our results suggest that fungal biomass typical in soil (<550 μg g-1soil) may create anoxic microniches and enable activity as well as dispersal of obligate anaerobes near hyphae in an otherwise inhabitable environment.
Dusny Christian、Harms Hauke、Kleinsteuber Sabine、Xiong Bi-Jing、Str?uber Heike、Wick Lukas Y.
Department of Solar Materials, Helmholtz Centre for Environmental Research ¨C UFZDepartment of Environmental Microbiology, Helmholtz Centre for Environmental Research ¨C UFZDepartment of Environmental Microbiology, Helmholtz Centre for Environmental Research ¨C UFZDepartment of Environmental Microbiology, Helmholtz Centre for Environmental Research ¨C UFZDepartment of Environmental Microbiology, Helmholtz Centre for Environmental Research ¨C UFZDepartment of Environmental Microbiology, Helmholtz Centre for Environmental Research ¨C UFZ
微生物学环境生物学生物科学研究方法、生物科学研究技术
Coprinopsis cinereaoxygenhyphaebacterial-fungal interactionsmycosphereplanar optodenanoparticlesphase-contrast microscopy
Dusny Christian,Harms Hauke,Kleinsteuber Sabine,Xiong Bi-Jing,Str?uber Heike,Wick Lukas Y..Impact of fungal hyphae on growth and dispersal of obligate anaerobic bacteria in aerated habitats[EB/OL].(2025-03-28)[2025-08-02].https://www.biorxiv.org/content/10.1101/2022.03.03.482870.点此复制
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