线性聚乙烯亚胺共聚物做为潜在MR可见基因载体

In order to improve transfection efficiency and confirm target delivery mechanism, stearic acid graft Poly(ethylene glycol)-linear Poly(ethylene imine)(SA-g-PEG-PEI) copolymer was synthesized and characterized by 1HNMR, FT-IR and GPC; SA-g-PEG-PEI used as co-delivery of SPIO and gene for potential magnetic resonance imaging monitoring gene delivery, SPIO loaded with various composition of copolymers of SA-g-PEG-PEI nanoparticls(NPs) were produced using an emulsion/solvent evaporation technique, the morphology investigated by TEM of blank and SPIO-loaded SA-g-PEG-PEI NPs were vesiclar; the diameter of all the NPs were about 150nm,however, the blank NPs were smaller; the zeta-potential of blank SA-g-PEG-PEI NPs was about +12mv, but all of them decreased compared to; the SPIO-loaded NPs of SA-g-PEG-PEI exhibited superparamagetic property at room temprature. All of the results prove SPIO-loaded NPs of SA-g-PEG-PEI can act as co-delivery MRI contrast and DNA carriers, provide carriers for MRI tracking gene delivery.