叶锈菌及激素诱导对小麦叶片中Ta-SKP2A-2基因表达的影响

In order to identify the signal pathway of F-box family Ta-SKP2A-2 genes in wheat resistance/susceptibility against leaf rust pathogen, the present study analyzed its expression profile in wheat leaves inoculated with different leaf rust strains and induced by 3 hormones. Results showed that the highest expression level of Ta-SKP2A-2 gene was observed in the combination of TcLr38 and 05-5-137③, the expression at 3h is 13 times higher than that of the control, while the induced expression is late (at 96 hpi) and lower(2 times higher than that of control) in other resistant combination of TcLr15 and 05-19-43②. In both compatible combinations, Ta-SKP2A-2 gene showed down regulation in combination of CS and 05-5-137③, and two peaks (3h and 96h) in TcLr15 inoculated with 05-5-137③. Results from hormones treatments showed that ABA can induce up-regulation of Ta-SKP2A-2, and IAA and MeJA can cause down regulation of the target gene in CS, while up-regulation of Ta-SKP2A-2 gene was mainly induced by IAA and ABA in TcLr15. Altogether, the whole tendency of Ta-SKP2A-2 gene was down regulation before 12hpi, which is same to that of IAA and MeJA. No similar expression profile was testified between inoculation with leaf rust pathogen and treatment of hormones in TcLr15.