白藜芦醇抑制辐射诱导的间充质干细胞IL-1β表达的机理研究

o investigate the effect of resveratrol on IL-1β production in Mesenchymal Stem Cells(MSCs) exposed to radiation, and explore the protective effect and mechanism of resveratrol for MSCs against radiation damage. Mesenchymal Stem Cells(MSCs) exposed to radiation, and explore the role of Sirt1 in radiation protection. Method MSCs were divided into control group, irradiation group and resveratrol group, and pretreatments of different dosage (50μM, 100μM, 200μM) of resveratrol were administrated to MSCs before radiation. The expression of IL-1β was detected using ELISA, western blotting and real time PCR after irradiation. shRNA silence Sirt1 gene transcription in MSCs, and detect the intracellular Sirt1mRNA expression to confirm the gene silencing; And treated with 200μM resveratrol to MSCs before radiation, the expression of IL-1β was detected using ELISA, western blotting and real time PCR after irradiation. Results The level of IL-1β secretion was decreased compared to radiation group (t=83.34, 24.48, 12.52, P<0.05), and meanwhile, the intracellular expression of IL-1β protein and mRNA of resveratrol group were also decreased significantly compared to radiation group （t=8.695,14.77,13.9; P<0.05）. The level of IL-1β secretion was increased compared to resveratrol group (t=18.57; P<0.05), and meanwhile, the intracellular expression of IL-1β protein and mRNA of RNAi combine with resveratrol group were also increased significantly compared to resveratrol group (t=10.24; P<0.05). Conclusion Resveratrol could inhibit the production of inflammatory factor IL-1β in MSCs induced by radiation. Sirt1 gene silence can obviously inhibit the radiation protective effect of resveratrol, it suggest that Sirt1 may play a key role in the process of inflammation induced by radiation.