辣椒遗传再生体系的研究和建立
Study and Establishment of Capsicum Annuum L. Genetic Regeneration System
选用3个辣椒品种（Capsicum annuum L.）的子叶和下胚轴，分别离体培养在附加不同激素的MS 培养基上，对苗龄、基因型、不同外植体、激素组合等对诱导外植体不定芽分化和芽伸长的影响进行研究。结果表明，苗龄对外植体不定芽分化有直接影响；子叶的不定芽分化率高于下胚轴；不定芽的分化率对基因型有很大的依赖性，GA3、AgNO3的添加能使芽伸长率提高。通过结果比较，筛选出了辣椒子叶和下胚轴离体再生的较好芽分化培养基为MS+1.0 mg/L ZT+0.2 mg/L IAA；芽伸长培养基为MS+1.0 mg/L ZT+0.2 mg/L IAA+ 2.0 mg/L GA3+5.0 mg/L AgNO3；生根培养基为 MS+0.1 mg/L IAA+0.2 mg/L NAA。
he cotyledon and hypocotyl explants of three chilli pepper cultivars were selected to culture on the MS medium with different hormones. .The effect of seedling stage,genotype, explant,hormone et al on the tissue culture from pepper explants was investigated. The results showed that seedling stage of explants had a direct impact on the shoot induction rate; shoot induction rate of cotyledons was higher than the hypocotyls; the genotype was very important to the shoot induction and elongation rate ,GA3、AgNO3 can improve the elongation rate. The best media for shoot induction is MS +1.0 mg / L ZT +0.2 mg / L IAA; shoot elongation medium is MS +1.0 mg / L ZT +0.2 mg / L IAA + 2.0 mg / L GA3 +5.0 mg / L AgNO3; rooting medium is MS +0.1 mg / L IAA +0.2 mg / L NAA.
王翊、王炳琴、陈国平、胡宗利、侯晓姝
遗传学植物学生物工程学
辣椒离体培养外植体植株再生
apsicum Annuum LIn vitro cultureExplantsRegeneration
王翊,王炳琴,陈国平,胡宗利,侯晓姝.辣椒遗传再生体系的研究和建立[EB/OL].(2008-11-05)[2025-08-10].http://www.paper.edu.cn/releasepaper/content/200811-122.点此复制
评论