反相高效液相色谱法有效分离和同时测定人体尿液中7种喹诺酮

Objective To establish a new method for effective separation and simultaneous determination of pipemidic acid and six fluoroquinolones in human urine by reversed phase high performance liquid chromatography with diode array detector. Methods The chromatographic separation was performed on ZY1104 C18 column (250 mm×4.6 mm, 5μm) at column temperature of 30 ℃. The mobile phase, a mixture of acetonitrile-0.02 mol/L tetrabuty lammonium bromide(v/v=9:91)– trifluoracetic acid buffer (pH 2.87), was delivered at a flow rate of 1.0 ml/min. The detection wavelength was 282 nm and the injection volume was 20 μl. Results The calibration curves for the seven quinolones were good linearity in the range from 0.5 μg/ml to 100 μg/ml with correlation coefficients of ≥ 0.9996. The detection limits were 0.036～0.088 μg/ml.The mean recoveries of the seven drugs ranged from 91.6% to 99.5% with relative standard deviations below 7% (n=5). Conclusion The method was convenient, rapid and accurate for effective separation and simultaneous determination of seven quinolones studied in human urine.