通络救脑注射液对Aβ致脑微血管内皮细胞损伤的保护作用

Objective To investigate the protective effects and mechanism of Tong Luo Jiu Nao（ TLJN）Injection and its active ingredients (including Geniposde and Ginsenoside Rg1), on human brain microvascular endothelial cells (BMECs) injured by β-amyloid (Aβ). Methods Human BMECs were pre-incubated by TLJN and Geniposde and GinsenosideRg1 for 2h, and then injured by Aβ. At different intervals，Cell Counting Kit-8 was employed to assay the activities of BMECs, the leakage of lactate dehydrogenase in the culture of BMECs was examined, and the expression levels of receptor for advanced glycation endproducts ( RAGE ) of BMECs was detected by Western blot method. Results TLJN Injection could significantly improve the activities of human BMECs injured by Aβ, decreased LDH leakage rate，and down-regulate the expression levels of RAGE. The two active ingredients showed different effects at different intervals. The effects of compound TLJN injection were obviously superior to those of two active ingredients. Conclusion TLJN injection has protective effects on the human BMECs injured by Aβ, and its mechanisms is related to the decrease of the expression of RAGE. TLJN injection has better effect than the active ingredients.