LncRNA ANCR对骨肉瘤细胞生物学特性的影响

ObjectiveTo investigate the impact of ANCR on biological behaviorofosteosarcoma cell line,including gowth,proliferation, invasion.Methods shRNA technology was used on reduction of osteosarcoma cell line U2OS, The biological behaviors were tested by MTT,Colony formation assay,Transwell and Matrigel assay in the osteosarcoma cells line U2OS with different ANCR expression level to define the function of ANCR. To investigate the cell cycles of U2OS with different ANCR expression level by Flow Cytometer for assessing the influence on osteosarcoma cell cycle. ResultsANCR knockdown was found to decrease the number of U2OS cells compared with the shConcells(P<0.01). Down-regulation of ANCR was found to reduce the number of viable U2OS cell colonies, implyingthat U2OS cellstreated by Lv-shANCR had a colony formation suppression ability compared with the control shRNA cells(P<0.01).The number of U2OS cells crossing the Matrigel coated ?lter in the shANCR group was prominently less than that in the shCon and con group(P<0.01).Inhibition of ANCR expression resulted that osteosarcoma cells were arrested in G0/G1 phase in the shANCR group compared with control group,the rate of G0/G1 was 61.6% (P<0.01).While, the percentages of S phase cells in the shANCR group were markedly decreased,and the rate of s phase was 21.2% compared with control group(P<0.01).Conclusions Decreased ANCR expression inhibited osteosarcoma cell,proliferation and invasion and promoted G0/G1 arrested in vitro，indicating that ANCR mayregulate the biological behavior of U2OS.