抗DNA抗体/阳离子质脂体复合物用于质粒DNA的定位递送

e reported for the first time a new type of plasmid DNA gene delivery system characterized by DNA-(anti-DNA) Antibody-Cationic lipid (DAC) micelles and permitted the highly efficient entrapment and delivery of plasmid DNA in vitro. The micelles were characterized with respect to size, charge, and DNA entrapment. DAC triplex system formed a stable suspension of nanospheres, with a mean particle diameter of around 360nm, and a zeta potential of –15mV. Fluorescent DNA studies demonstrated greater cell uptake in vitro with DAC compared to the various control formulations. Confocal microscopy studies confirmed nuclear entry in A10 cells with DAC, while formulations without the antibody demonstrated no nuclear entry. Cell culture studies revealed that DAC gave 4-fold greater level of transfection than the formulation without the antibody. It is concluded that anti-DNA antibodies can markedly enhance plasmid DNA gene transfer in A10 cells in culture. This effect appears to be strongly associ