烯丙基荧光素-丙烯酰胺沉淀共聚荧光分子印迹选择性荧光检测三氟氯氰菊酯残留

fluorescent molecularly imprinted polymer (FMIP) sensor is fabricated via precipitation polymerisation. The MIP sensor is prepared upon copolymerisation of acrylamide with a small quantity of allyl fluorescein in the presence of LC to form recognition sites. The as-synthesised microspheres exhibited spherical shape, high fluorescence intensity and highly selective recognition. Under optical conditions, polymer microspheres are successfully applied to selectively and sensitively detect LC, and a linear relationship described by the Stern-Volmer equation (I0/I) - 1 = 0.29oCC - 0.00608 with a correlation coefficient of 0.9936. A lower limit of detection (LOD) is found to be 0.004 nM. The results of practical detection suggested that the developed method is satisfactory for determination of LC in honey samples. The FMIP sensor has certified that they can be reused for not less than 5 times without remarkably loss of signal intensity.