林木体胚发生中胚性培养细胞的cDNA文库构建策略
Strategy on construction of cDNA library in trees somatic embryogenesis
体细胞胚胎发生在林木的繁殖，遗传改良等领域有很大的应用价值。在研究体细胞胚胎发生机理方法中，cDAN文库构建是发现新基因和研究基因功能的基本工具。从cDAN文库中可以筛选到目的基因，并直接用于该目的基因的表达。本文介绍了林木体胚发生中几种cDAN文库的原理和优缺点及其构建策略。
Somatic embryogenesis is of great value in various sides such as seeding propagation, genetic improvement in trees. In the research of trees somatic embryogenesis mechanism method , cDNA library construction is basic tool of finding new genes and researching gene function. Targe genes can be selected from cDNA library, and used to analyze the expression of the genes. The paper mainly explains several methods of cDAN library construction, their basic principles, advantages and disadvantages in trees somatic embryogenesis.
施季森、吴淳、陈金慧、李婷婷
分子生物学植物学生物科学研究方法、生物科学研究技术
体细胞胚胎发生cDAN文库胚性细胞
somatic embryogenesiscDNA libraryembryogenic cell
施季森,吴淳,陈金慧,李婷婷.林木体胚发生中胚性培养细胞的cDNA文库构建策略[EB/OL].(2010-01-25)[2025-08-16].http://www.paper.edu.cn/releasepaper/content/201001-1025.点此复制
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