Ru(bpy)32+-(CH2)7-MV2+合成及其光切割DNA研究
Synthesis and Photocleavage of DNA by Ru(bpy)32+-(CH2)7-MV2+
本文将电子受体MV2+通过饱和柔性碳链引入到光敏体三联吡啶钌结构中，合成得到新化合物Ru(bpy)32+-(CH2)7-MV2+。利用其分子内高效专一的电子转移特性，研究了它对pBR322质粒DNA的光敏切割活性，通过使用DTT，组氨酸，DMSO、乙醇和甲酸钠等活性氧捕捉剂，充分证明其光敏切割机理为电子转移机理，八元瓜环(Cucurbit[8]uril, CB8)与配合物1的自组装可以有效抑制电子回传，使自组装化合物对DNA的切割能力得到提高。
Ru (II) complex covalently linked with viologen units, Ru(bpy)32+-(CH2)7-MV2+, was synthesized and characterized. Ru(bpy)32+-(CH2)7-MV2+ was found to cleave plasmid DNA strand effectively under irradiation by visible light, due to the photosensitized intramolecular electron transfer from the Ru(bpy)32+ to viologen moieties, the photocleavage of plasmid DNA was observed both in air and under Ar atmosphere. The inclusion between 1 and cucurbit[8]uril can inhibit the backward electron transfer in 1 and then enhance the photocleavage efficiency.
和艳霞、孙世国、彭孝军、樊江莉、杨志刚
生物化学生物物理学分子生物学
分子内电子转移钌配合物B[8]自组装电子回传NA光切割
Intramolecular Electron TransferRuthenium (II) ComplexInclusion of CB[8Backward Electron TransferNA Photocleavage
和艳霞,孙世国,彭孝军,樊江莉,杨志刚.Ru(bpy)32+-(CH2)7-MV2+合成及其光切割DNA研究[EB/OL].(2009-04-29)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/200904-908.点此复制
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