淫羊藿甙诱导滑膜间充干细胞向成骨细胞分化实验研究

Objective: To explore characteristics of Epimedium glycoside inducing synovial mesenchymal stem cells (SMSCs) differentiating into osteoblasts. Methords: The fourth generation of SMSCs were seeded in 5 6-well plates by 5 × 106 ,each plate was divided into 3 groups, blank group, control group and Epimedium glycoside group,each group had 2 holes, two days later the culture medium was changed with inducing fluid. The blank group was added L-DMEM medium containing 10% fetal bovine serum (FBS), the control group was added inducing agent on the basis of the L-DMEM medium (containing β glycerophosphate 10mmol / L, dexamethasone 0.1 umol / L, vitamin C 50mg / L) ,the Epimedium glycoside group was added L-DMEM medium containing 40% Epimedium glycoside. On the 14th day removed a 6-well plate made alkaline phosphatase staining; on the 21st day one of the remaining 6-well plates was made alizarin red staining, the rest three plates was made alkaline phosphatase, type I collagen and osteocalcin immunohistochemical analysis respectively .Results: cellular morphology changed from spindle into oval shape after SMSCs induced by osteogenic inducer and Epimedium glycoside, cell volume increased, on 14th day granular substance was visible on the surface of cell colony, alkaline phosphatase staining was positive, 13 days later white calcium nodules was visible, Alizarin red staining was positive on 21st day ,also alkaline phosphatase ,type I collagen and osteocalcin immunohistochemical staining positive, showing the characteristics of osteoblasts. Conclution: Epimedium glycoside was able to promote differentiation of SMSCs into osteoblast.?????