GF-β1诱导下人肝星状细胞糖蛋白糖谱的研究

he hepatic fibrogenesis is a kind of disease happens generally, which may develop to hepatic cirrhosis if it couldn’t been diagnosed and treated promptly. The human Hepatic stellate cells (HSCs) activation and proliferation are the center links which the hepatic fibrogenesis forms.At present many research aim at HSCs are mainly in the field of gene,the glycan profile research very little involves. Method: The experiment cultured LX-2 cells in the presence of 2 ng/mL TGF-β1 for 24h.Semi-quantitative RT-PCR examined the LX-2 cell line whether had the hepatic fibrogenesis change.The total proteins of the induced LX-2 cells and its control were extracted, labled with Cy3 fluorescent dye, then incubated with the lectin microarray. Glycoprotein Glycan profiles were analysed to screen differently expressed Glycans, and get the relations of glycoprotein glycan and hepatic fibrogenesis disease. Conclusion: TGF-β1 could be used to induce LX-2 cells; The lectin microarray could give the “quiescent” LX-2 cells and the “activated” LX-2 cells induced by TGF-β1 glycoprotein glycan profiles and differences in glycan-profiles.