一株猪传染性胃肠炎病毒的S基因进化与细胞致病性分析

member of alphacoronavirus 1, porcine transmissible gastroenteritis virus was isolated from Shanghai, China. It is designed TGEV ZH. Here, its full-length of S gene was sequenced and compared with S genes from other TGEV, or canine coronavirus (CCoV) and feline coronavirus (FCoV) isolates. Results showed that TGEV ZH had high similarity (99.8%) with TGEV HN2002, a Chinese isolate, and both were at the TGEV miller cluster on the phylogenetic tree. Variation of S gene was mainly distributed in RBD region, but in C- is less than in N- RBD region. The recombination detection program 3.44 showed that the S RBD of TGEV ZH might be a recombinant of N-RBD of TGEV and C-RBD of CCoV, which indicated the sequence between N-RBD and C-RBD may be a recombinant "hot spot" for TGEV, CCoV, and FCoV isolates. Twenty six of N-glycosylation sites on S protein of TGEV ZH were predicted, among of them, three sites were different from TGEV purdue and ten sites were different from those of CCoV 1-71 or FCoV 79-1683. Furthermore, the replication and cytopathogenesis of TGEV ZH in porcine ST cells, canine A72 cells, feline FCWF cells were analyzed. Results showed both TGEV ZH and purdue replicated well and caused apoptosis in ST and A72 cells, but in FCWF cell only purdue but ZH. Meanwhile CCoV 1-71 and FCoV 79-1683 could replicate in A72 and FCWF cells, but not in ST cells. We assumed that the difference of virulence and tropism among TGEV, CCoV, and FCoV isolates may be associate with the diversity of N-glycosylation sites in RBDs.