母猪日粮蛋白水平对断奶仔猪脂肪沉积的影响及其机制研究

Sows in control group were fed with diets containing 12% and 14% of protein during pregnancy and lactation respectively, whereas protein levels reduced by half in treated group. Piglets born to sows in both control and treated groups were sacrificed after weaning. Back fat thickness of weaned piglets was measured. The leptin concentration in subcutaneous fat was determined by RIA assay. Gene expression of leptin, LepR, HSL, ATGL and FAS were quantitated with relative quantitative real time RT-PCR.We used western blot analysis to detect the content of perilipin and phosphorylated perilipin.The activity of lipases was also determined. The results showed that the body weight and back fat thickness of piglets in treated group were significantly smaller than that in control group. The leptin concentrations demonstrated a decreased tendency in maternal protein restriction group. Piglets in treated group exhibited significantly lower expression of ATGL, leptin, FAS mRNA (P < 0.05). The phosphorylated perilipin content displayed a promoted trend in MPR offspring piglets than that in control piglets. Besides, the activity of lipases in subcutaneous fat was sigficanlty higher in MPR piglets than that in control group. The results suggest that the effect of maternal dietary protein restriction on fat deposition acted not only by reducing fat synthesis, but also by improving the ability of lipolytic.