复方浙贝颗粒联合阿霉素对AML-KG-1a移植瘤PI3K/AKT信号通路影响

Objective：To investigate the effect of Compound Zhe Bei granule(CZBG)combined with doxorubicin on the expression of mTOR ,PI3K,NF-κB and PTEN in KG-1a cell line transplantation tumors in Mice．Methods：Subcutaneous tumor xenograft model is established by injecting the acute myeloid leukemia cell line KG-1a in the axillary flank of BALB/c-nu nude mice．Drug-combination of CZBG intragastric administration and doxorubicin intraperitoneal injection (i.p.) is given to the BA LB/c-nu nude mice．The tumor xenografts are made into slice after the dissection, and the expression of mTOR ,PI3K,NF-κB and PTEN in KG-1a cell line tumor xenografts are detected by immunohistochemical technique. The optical density(OD) of mTOR ,PI3K,NF-κB and PTEN in KG-1a cell line tumor xenografts are measured by Image PmPlus6.0．Results: Compared with ADM group , high-doseage CZBG can inhibit the mTOR expression obviously(P＜0.05) ，mid-doseage and high-doseage CZBG can both inhibit the NF-κB expression（P＜0.05) ．Conclusion: CZBG can collaborate with doxorubicin to reduce the expression of mTOR and NF-κB.