受照脑胶质瘤细胞对神经干细胞诱导的旁效应

he rapid development of radiotherapy technology significantly increases the overall survival of patients with brain tumors after radiotherapy. However, the brain injury caused by ionizing radiation may seriously reduce the long-term quality of life of cancer patients after treatment, thus has attracted many researchers\' attention, such as cognitive dysfunction caused by cranial irradiation. Inhibition of neurogenesis by radiation has been found to be an important mechanism underlying radiation-induced cognitive impairment. Neural stem/progenitor cells existing in a microenvironment composed of many types of cells are vulnerable to the influence from other cells. And the occurrence of radiation-induced bystander effects can cause biological changes in unirradiated cells. However, whether radiation-induced bystander effects contribute to the inhibition of neurogenesBystander Effects Induced by Irradiated Glioma Cells on Neural Stem Cellsis is rarely reported so far. In this study, using co-culture method, we found that after co-culture with glioma cells exposed to X-rays, neural stem cells (NSCs) displayed significantly inhibited proliferation, neurosphere formation, differentiation and stemness maintenance ability, indicating an occurrence of bystander effects in NSCs induced by irradiated glioma cells. These results suggest that irradiated cancer cells may inhibit neurogenesis through decreasing the proliferation, stemness and differentiation of unirradiated NSCs via bystander effects, thus contributing to the development of radiation-induced cognitive decline.