基于冷冻电镜三维重构技术的Bm-CPV结构与转录机制研究

s the simplest single-layer capsid dsRNA virus of Reoviridae family, cytoplasmic polyhedrosis virus (CPV)-the representative virus-is always the most significant point and has great significant in Reoviridae virus study. By cryo-electron microscopy and three dimension reconstruction, the structure of the CPV have been determined: a icosahedral partical with twelve vertices, and there are ten transcriptional enzyme complexes(TECs) organized with ten dsRNA in a specific. Capsid is consists of sixty asymmetric unit. Asymmetric unit contains one turret protein(TP), two large protrusion protein(LPP) and two capsid shell protein(CSP). Turret is consist with five channels formed with TP, and each TP contains three active site domains. When MT-2 binding a S-adenosyl-L-methionine(SAM), the virus capsid expand and active the transcription process, and the nascent mRNA will across the channel and begain the capping process. This review summarizes some professional papers about CPV, and introduce the mechanism of CPV structure, transcription and capping process，hope can help other researchers in their study of the CPV.