甘薯长喙壳病菌对三种寄主的致病性与ITS分析

It was recorded that Ceratocystis fimbriata was a host-specialization.This experiment aims to study pathogenicity of Ceratocystis fimbriata on pomegranate, sweet potato and taro, with pricking and dipping root method to original/cross host inoculation. And do rDNA-ITS sequence analysis as well. The results showed that the isolates have the pathogenicity to their three own host respectively; The isolates from sweet potato can not infect that pomegranate and taro with host -specialization. However, The isolates from pomegranate and taro can infect each other. The ITS(internal transcription spacer )primer pair ITS1 and ITS4 were used and amplified the fragment around 600bp. Analysis of the ITS sequence data showed that the isolates were 100% homologous between taro and pomegranate isolates, but 97% homologous from sweet potato to both taro and pomegranate isolates by BLAST analysis. The comparison results by DNAMAN showed that the taro and pomegranate isolates almost the same, more difference between sweet potato to taro and pomegranate isolates.