抗菌肽基因转化大豆的研究

antimicrobial peptide gene was transformed into soybean via agrobacterium-mediation. PCR amplification and expression of green fluorescent protein was used to detect transgenic soybean plants. The 60 mg/L kanamycin could effectively filter the transformed plants, and 200 μmol/L acetosyringone in coculture medium was benefit for improving the gene transduction efficiency. The kanamycin in rooting medium is harmful to shoots root. The PCR amplification and and expression of green fluorescent protein proved that the antimicrobial peptide genes has been transformed into soybean and expressed.