全豆粉对高脂日粮饲喂小鼠肝脏脂质代谢作用的研究

his paper was designed to investigate the beneficial effects of the whole soybean powder, compared with the processed soybean powder(soybean milk without okara in traditional soybean production), on the lipid metabolism in C57BL/6J mice fed with a high-fat(HF) diet. The mice were randomly divided into four groups: control group (AIN-93 M), high-fat group (HF), high-fat + powdered soybean(HFS) and high fat + powered whole soybean (HFWS).The mice were sacrificed after eight weeks and the concentration of total cholesterol (TC), triglyceride (TG) and high density lipoprotein cholesterol (HDL-C) in serum were measured. Liver histological analysis was performed by microscopy. The mRNA expression of SREBP-2, CYP71a, LXRα and HMGCRin liver were measured by RT-PCR. Compared with the high-fat diet group, the body weight, serum TC, TG level of HFWS group were significantly decreased (P<0.05), while atheroclerosis index (AI) was significantly increased (P <0.05). RT-PCR result showed that the expression of CYP71a and LXRα were significantly down-regulated, the expression of SREBP-2 and HMGCR were up-regulated in HF group. Compared with HF group, HFWS and HFS were significantly increased the mRNA expression of LXRα and CYP7a1 (P <0.05), decreased the expression of SREBP-2 and HMGCR (P <0.05). The results implied that the whole soybean have more beneficial effects on the control of serum lipid than the processed soybean. It is possible that whole soybean is rich of dietary fiber than processed soybean.