赛鸽及食用鸽视网膜的转录差异分析
ranscriptional analysis on retina of racing pigeon and meat pigeon
人们认为鸽子等鸟类具有一定的地磁导航能力,但实际上只有人工培育和训练的赛鸽才具有较强归巢行为和导航能力,而普通食用鸽并不能导航归巢。行为学实验表明在红光条件下包括鸽子在内的许多鸟类失去了感受磁场的能力。通过转录组技术研究比较正常赛鸽和红光条件下饲养的赛鸽的视网膜基因表达差异,同时以普通食用鸽作为参照,筛选出相关的基因37条,利用基因本体数据库和KEGG数据库分析差异基因功能和所在的信号通路,并用反转录PCR的方法检测差异基因在鸽子主要组织的表达情况。研究发现,差异基因富集于细胞黏附,新陈代谢等通路,说明红光处理并非仅是对磁受体的光感受功能有影响,还可能对鸽子视力造成较大影响。
It is believed pigeons are can use geomagnetic field information in the navigation process. Usually, only the artificial cultivation and training of pigeons have a strong homing behavior and navigation capabilities, and ordinary edible pigeons can't navigate homing. Behavioral experiments show that pigeon lost their ability to sense magnetic fields under red light. The transcriptome gene was used to study the difference of retinal gene expression between athletic pigeon and red light conditions athletic pigeon, while the common pigeon used to reference. 37 genes were screened, the gene function and the signal pathway were analyzed by the gene ontology database and Kyoto Encyclopedia of Genes and Genomes database. Reverse transcription PCR was used to detect the expression of different genes in the main tissues of pigeons. The study found that differences in gene enrichment in cell adhesion, metabolism and other pathway, indicating that red light treatment of pigeons may have a greater impact.
王文浩、景成宇、刘志明、吴文健
分子生物学生理学动物学
鸽子地磁导航视网膜转录组
pigeonretinatranscriptomegeomagnetic navigation
王文浩,景成宇,刘志明,吴文健.赛鸽及食用鸽视网膜的转录差异分析[EB/OL].(2017-06-30)[2025-08-24].http://www.paper.edu.cn/releasepaper/content/201706-279.点此复制
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