The IncRNA APOLO interacts with the transcription factor WRKY42 to trigger root hair cell expansion in response to cold
The IncRNA APOLO interacts with the transcription factor WRKY42 to trigger root hair cell expansion in response to cold
ABSTRACT Plant long noncoding RNAs (IncRNAs) have emerged as important regulators of chromatin dynamics, impacting on transcriptional programs leading to different developmental outputs. The lncRNA AUXIN REGULATED PROMOTER LOOP (APOLO) directly recognizes multiple independent loci across the Arabidopsis genome and modulates their three-dimensional chromatin conformation, leading to transcriptional shifts. Here, we show that APOLO recognizes the locus encoding the root hair (RH) master regulator ROOT HAIR DEFECTIVE 6 (RHD6) and controls RHD6 transcriptional activity leading to cold-enhanced RH elongation, in association with the Polycomb Repressive Complexes (PRC) 1 and 2. Additionally, we demonstrate that APOLO interacts with the transcription factor WRKY42 and modulates its binding to the RHD6 promoter. WRKY42 is required for the activation of RHD6 by low temperatures and WRKY42 deregulation impairs cold-induced RH expansion. Collectively, our results indicate that a novel ribonucleoprotein complex involving APOLO and WRKY42 forms a regulatory hub which activates RHD6 by shaping its epigenetic environment and integrates different signals governing RH growth and development.
Moison Micha?l、Pacheco Javier Mart¨anez、Lucero Leandro、Fonouni-Farde Camille、Benhamed Moussa、Iba?ez Fernando、Ariel Federico、Rodr¨aguez-Melo Johan、Estevez Jos¨| M.、Bazin J¨|r¨|mie、Christ Aur¨|lie、Crespi Martin
Instituto de Agrobiotecnolog¨aa del Litoral, Universidad Nacional del Litoral, CONICET, FBCB, Centro Cient¨afico Tecnol¨?gico CONICET Santa FeFundaci¨?n Instituto Leloir and IIBBA-CONICETInstituto de Agrobiotecnolog¨aa del Litoral, Universidad Nacional del Litoral, CONICET, FBCB, Centro Cient¨afico Tecnol¨?gico CONICET Santa FeInstituto de Agrobiotecnolog¨aa del Litoral, Universidad Nacional del Litoral, CONICET, FBCB, Centro Cient¨afico Tecnol¨?gico CONICET Santa FeInstitute of Plant Sciences Paris-Saclay (IPS2), CNRS, INRA, University Paris-Saclay and University of Paris Batiment 630Instituto de Investigaciones Agrobiotecnol¨?gicas, CONICET, Universidad Nacional de R¨ao CuartoInstituto de Agrobiotecnolog¨aa del Litoral, Universidad Nacional del Litoral, CONICET, FBCB, Centro Cient¨afico Tecnol¨?gico CONICET Santa FeInstituto de Investigaciones Agrobiotecnol¨?gicas, CONICET, Universidad Nacional de R¨ao CuartoFundaci¨?n Instituto Leloir and IIBBA-CONICET||Centro de Biotecnolog¨aa Vegetal (CBV), Facultad de Ciencias de la Vida (FCsV), Universidad Andr¨|s Bello, Santiago, Chile and Millennium Institute for Integrative Biology (iBio)Institute of Plant Sciences Paris-Saclay (IPS2), CNRS, INRA, University Paris-Saclay and University of Paris Batiment 630Institute of Plant Sciences Paris-Saclay (IPS2), CNRS, INRA, University Paris-Saclay and University of Paris Batiment 630Institute of Plant Sciences Paris-Saclay (IPS2), CNRS, INRA, University Paris-Saclay and University of Paris Batiment 630
分子生物学植物学遗传学
root hairscell expansionlong noncoding RNAsAPOLOtranscription factorsRHD6WRKY42cold
Moison Micha?l,Pacheco Javier Mart¨anez,Lucero Leandro,Fonouni-Farde Camille,Benhamed Moussa,Iba?ez Fernando,Ariel Federico,Rodr¨aguez-Melo Johan,Estevez Jos¨| M.,Bazin J¨|r¨|mie,Christ Aur¨|lie,Crespi Martin.The IncRNA APOLO interacts with the transcription factor WRKY42 to trigger root hair cell expansion in response to cold[EB/OL].(2025-03-28)[2025-08-02].https://www.biorxiv.org/content/10.1101/2020.07.13.188763.点此复制
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