基于Fe2O3@Au磁性核壳纳米粒子的抗原分离及表面增强拉曼光谱检测

Fe2O3@Au core@shell nanoparticles were prepared by the reduction of Au3+ onto the surfaces of Fe2O3 nanoparticles and characterized by scanning electron microscopy (SEM). The results revealed that the prepared core/shell nanoparticles were covered by Au shell and the surfaces of the core/shell particles are jagged after the initial addition of Au3+. Surface enhanced Raman scattering (SERS) activities of these magnetic nanoparticles were studied by using pyridine (Py) as a probe molecule after a magnet was used to concentrate the colloid. It was found that the SERS intensity depends on the Au shell thickness of the core/shell nanoparticles and strengthens with the increasing shell thickness. The detection limit for Py can be very low by using the magnetic Fe2O3@Au shell nanoparticles and even down to 10-7 M. This Fe2O3@Au magnetic core@shell nanoparticles exhibited excellent effect for the separation of antigen in solution.