苦马豆素对新生SD大鼠大脑皮质神经细胞形态学损伤作用的研究

In order to observe the impact of swainsonine in cortical neurons of neoatal SD rats, in the experimental group, the model of primary cultures of neonatal SD rats within cerebral cortex nerve cell was built. Viability of the neurons was investigated using MTT method. Determination of swainsonine on rat cerebral cortical neurons exposed to IC50 by Karber. Neurons were given swainsonine, respectively, at different concentrations for 12 hours. The morphological changes of neurons were observed and the pericaryon and processes were measured by inverted light microscope. The highest viability was on 5 days. The results indicated that the growth of neurons was inhibited by swainsonine in vitro, with an IC50 value of 0.8511 mg/ml. In comparison with the control group, except the 0.5mg/ml of swainsonine, density of neurons and neural network decreased in poisoning groups (P<0.05), the pericaryon and processes of neurons significantly decreased (P< 0.05) but the difference was not significant in part groups (P>0.05); With increasing concent rations of swainsonine, there was a reductive trend morphological changes of neurons in comparison with the control group, The relation of dose-effect was obviously observed between swainsonine and morphological changes.