mRNA差异显示技术及其研究进展

mRNA differential display was established by Liang and Pardee in 1992 for the purpose of displaying the mRNA differences between two tissues. The technique makes it possible to detect the genes differential displayed between specified cells and tissues at the level of mRNA. All the mRNA changes could be displayed side by side as cDNA band differences by mRNA differential display and the genes corresponding to these differential mRNA could be obtained. Most cell activities with changes at the transcriptional level can be investigated and, thus, it can be applied to addressing such topics as cell differentiation, development, cell cycle and response of cells to their environment. The paper mainly focuses in the principle, advantages, disadvantages, modification, application and prospects of the mRNA differential display.