大豆11S蛋白/刺槐豆胶溶液共混体系冷致凝胶流变及微结构性质研究
Research on rheology and microstructure of the cold-set soybean glycinin/LBG blend gels
采用相图分析、动态流变及激光共聚焦(CLSM)技术研究了葡萄糖酸内酯(GDL)冷致诱导大豆11S蛋白(Gly)/刺槐豆胶(LBG)共混体系凝胶的动态粘弹性质及微结构。结果表明: 随孵化时间的延长,凝胶粘弹性模量增加,微结构中多糖相占位面积呈先增加而后基本保持不变趋势;随LBG浓度(0.05%-0.35%,w/v)的升高粘弹模量呈现先上升后下降的趋势,伴随孵化终点的凝胶微结构由蛋白单连续相体系→蛋白多糖双相体系→多糖连续相体系的转变。冷致共混凝胶强度随着Gly蛋白浓度(2.0%-6.0%,w/v)、孵化温度(15℃-45℃)及GDL添加量(0.25%-0.55%,w/v)提高凝胶强度也呈先上升后下降趋势,共混凝胶微结构保持为的蛋白单连续形貌。共混凝胶的流变和微结构取决于体系的相分离与凝胶过程的竞争结果,其多样性为构建控释功能成分的食品凝胶材料提供了应用基础。
he rheological behavior and microstructure of glucono-δ-lactone (GDL) induced cold-set soybean glycinin(Gly)/locust bean gum (LBG) mixed gels was studied with phase diagram, dynamic rheology and confocal laser scanning (CLSM) analysis technology. With gelation incubation time prolonged the viscoelastic modulus of Gly/LBG(4.0%/0.25%,w/v) blend gel were increased and LBG phase occupancy area was first increased and then keep constant almost. The viscoelastic modulus of Gly/LBG was first increased and then decreased with LBG concentration increases(0.05%-0.35%,w/v), while the gel microstructure was changed from single Gly continuous to Gly-LBG bicontinuous and then into a single polysaccharide continuous. The viscoelastic modulus of Gly/LBG was also first increased and then decreased with Gly concentration (2.0%-6.0%,w/v),gelation incubation temperature (15℃-45℃) or GDL addition amount (0.25%-0.55%,w/v) increases, coupling with microstructure keep single Gly continuous. The rheological and structural diversity of the Gly/LBG blend gel depends on the competition results between phase separation and gelation, which provides application possibility of novel cold-set gels for practical use in functions component controlled release.
闵维、杨晓泉、朱建华
生物化学生物物理学生物工程学
大豆11S蛋白刺槐豆胶凝胶流变微结构
soybean glycininlocust bean gumrheological propertiesmicrostructuregelation
闵维,杨晓泉,朱建华.大豆11S蛋白/刺槐豆胶溶液共混体系冷致凝胶流变及微结构性质研究[EB/OL].(2014-01-24)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/201401-1126.点此复制
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