蒺藜苜蓿SLs受体MtD14功能研究

Strigolactones/SLs is an important plant hormone that regulates plant growth and development. Its receptor D14 is an α/β hydrolase, which not only senses SLs, but also acts as a hydrolase to hydrolyze SLs and transmit downstream signal transduction, which is a key component in the SLs signal pathway. Medicago truncatula has the advantages of self-pollination, small genome, mature and stable genetic transformation technology, and is an excellent model leguminous plant. In order to explore the effect of SL receptor MtD14 on the growth and development of Medicago truncatula, the sequence of MtD14 was analyzed and the physicochemical properties of MtD14 were studied. Moreover, through the statistics of mutant plant phenotype, it was found that MtD14 deletion would lead to the decrease of plant height and increase of branching. Finally, MtD14 overexpression genetic material was cFunction of SLs receptor MtD14 in Medicago truncatulareated through agrobacterium-mediated genetic transformation, which provided an important basis for further investigation of the biological function of MtD14.