凝集素磁性微粒的制备，表征及其应用
Preparation, Characterization and Application of Lectin-Magnetic Particle Conjugates
【目的】制备一种可以用于快速、简便分离各种生物样品中糖蛋白的凝集素磁性微粒复合物，用于糖蛋白质组学的研究。【方法】利用制备的环氧化磁性微粒复合物，通过氨基或者巯基同磁性微粒上的环氧基团的化学反应，将凝集素固定化至磁性微粒的表面，并通过各种表征方法对该反应进行优化。【结果】通过条件优化，制备出了平均粒径为3.50 μm，比表面结为96.29 m2/g且富含环氧基团的磁性微粒。利用该磁性微粒制备的凝集素Con A磁性微粒可用于血清中糖蛋白的分离，并具有很好特异性和重复性。【结论】本方法制备的凝集素磁性微粒能很好的分离生物样品中的糖蛋白，可用于糖蛋白质组学的研究。
Magnetic particles have been developed for various applications in the biological and clinical determination. In this study, we synthesized the epoxy-activated magnetic particles to immobilization the lectins. The epoxy-activated magnetic particles were the superparamagnetic supports with a specific surface area of 96.29 m2/g. The each gram of the magnetic particles can be saturated with 186.31±5.16 mg Con A. The lectin-magnetic particle conjugates can isolate glycoproteins specially via the lectin's carbohydrate recognition domain with well reproducibility. This method can be a good tool to be applied in the glycoproteomics research.
杨刚龙、李铮
生物化学生物工程学分子生物学
生物化学和分子生物学环氧化磁性微粒复合物凝集素磁性微粒复合物糖蛋白
Biochemistrty and molecular biologyExpoxy-activated magnetic particlesLectin-magnetic particle conjugatesGlycoprotein
杨刚龙,李铮.凝集素磁性微粒的制备，表征及其应用[EB/OL].(2013-01-23)[2025-08-19].http://www.paper.edu.cn/releasepaper/content/201301-990.点此复制
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