抗蓝耳病shRNA转基因表达载体的构建与功能验证
onstruction and functionality test of transgenic vector expressing shRNA targeting against PRRSV
本试验将靶向猪繁殖与呼吸综合征病毒（PRRSV）的shRNA表达盒插入pEGFP-N1的EcoO109I酶切位点构建重组转基因载体pEGFP-G1，并在转基因Marc-145细胞验证其抑制PRRSV复制的功能。用pEGFP-G1转染Marc-145细胞24 h后感染欧洲型PRRSV，病毒感染后每天观察细胞病变（CPE）情况。结果表明本试验所获pEGFP-G1可明显抑制PRRSV复制。
he purpose of this study was to construct recombinant transgenic vector pEGFP-G1 by shRNA expression box targeting against PRRSV inserted into the EcoO109I site of pEGFP-N1 and the functionality test of the resulting pEGFP-N1 inhibiting virus replication were conducted in Marc-145 cells．24 h after transfection by pEGFP-N1，cells were infected with PRRSV（Europe）and CPE were observed daily．The result showed that PRRSV replication could be inhibited by pEGFP-G1 in MARC-145 cells significantly as compared to the controls．
罗碧平、剧世强、马汝钧、芮荣
分子生物学遗传学生物工程学
猪生殖与呼吸综合征病毒shRNA质粒构建细胞病变
PRRSVshRNAplasmid constructioncytopathic effect
罗碧平,剧世强,马汝钧,芮荣.抗蓝耳病shRNA转基因表达载体的构建与功能验证[EB/OL].(2012-06-12)[2025-06-07].http://www.paper.edu.cn/releasepaper/content/201206-178.点此复制
评论