电穿孔法在衣藻叶绿体转化中的应用

hlamydomonas reinhardtii was an important model organism in the world of eukaryotic cell chloroplast biology. Up to now, several protocols can mediate the transformation in C.reinhardtii, include glass beads, Agrobacterium tumefaciens, electroporation and particle bombardment system. Electroporation is the most efficient method for nuclear genome transformation. However, the stable transformation of C.reinhardtii chloroplast was long hampered by non-efficient methods to deliver foreign DNA across the cell wall and three membranes. At the same time, the recipient cells were not caused severely mechanical damage for the manipulation. In this research, we introduced a construct into recipient cell by electroporation and established a simple high-efficiency strategy for the transformation of C. reinhardtii chloroplast. Our data indicated electroporation is a simple method for high-frequency chloroplast transformation of Chlamydomonas.