多功能纳米载体CDP/CDPF用作基因载体功能的研究

Objective:To explore the biocompatibility and transfection ability of multi-functional nano carriers of CDP and CDPF. Methods:143B cell line was used for research.Using Gel retardation assay to detect CDP and CDPF composite capability of AEG-1 siRNA, MTT assay was used to detect CDP and CDPF toxicity in 143B cells, the ability of AEG-1 of CDP and CDPF to carry siRNA was detected by fluorescence inverted microscope and flow cytometry. Results:Both CDP and CDPF started to form complete complexes with siRNA at an N/P ratio of 8; Even at high concentration of CDP and CDPF complexes (100 μg/mL), showed no obvious negative effect on cell viability; Cells incubated with CDPF/siAEG-1 (N/P:20) complexes exhibited almost similar efficiency (98.7%± 3.1%) of transfection compare to Lipofectamine 2000/AEG-1 siRNA complexes (97.1%± 3.8%). Conclusions: CDP and CDPF could be used as a no obvious cytotoxicity small interference RNA transfer vector and high efficient non-viral vector for gene delivery to143B cells.