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Early-life feeding accelerates gut microbiome maturation in piglets

Early-life feeding accelerates gut microbiome maturation in piglets

来源:bioRxiv_logobioRxiv
英文摘要

ABSTRACT Early-life microbiome perturbations have been suggested to have important effects on host development, physiology, and behaviour, which can persist throughout life. We hypothesise that early feeding (access to a pre-weaning fibrous diet) can affect gut microbiome colonisation and development in neonatal piglets. In this longitudinal study, a customised fibrous diet was provided to early-fed piglets (EF; 6 litters) starting two days after birth until weaning (28 days of age) in addition to mother’s milk, whereas control piglets (CON; 4 litters) suckled sow’s milk only. Rectal swabs were collected at multiple timepoints until six weeks of age (i.e., 2 weeks post-weaning) to investigate intestinal microbiota composition development over time using 16S rRNA gene profiling (n = 10 piglets per treatment). We observed a dynamic intestinal microbiota colonisation pattern during the pre-weaning period in both treatment groups, which rapidly stabilised within 2 weeks post-weaning. The microbial (alpha) diversity increased with age and seemed to reach a plateau in the early post-weaning time-point (day+5). The homogenous post-weaning microbiota was represented by microbial groups including Prevotella, Roseburia, Faecalibacterium, Ruminococcus, Megasphaera, Catenibacterium and Subdoligranulum. Remarkably, early feeding of neonatal piglets resulted in accelerated maturation of the intestinal microbiota at pre-weaning time-points, characterised by increased rate of microbial diversity and expanded colonisation of typical post-weaning associated microbial groups (mentioned above) at pre-weaning stages. The acceleration in EF piglets was illustrated by the simultaneous emergence of typical post-weaning-associated microbial groups and a more rapid decline of typical early-life/pre-weaning microbial genera. In addition, the individual eating behaviour scores of the piglets quantitatively correlated with the accelerated change of their microbiome. Overall, these findings show the importance of early-life nutritional strategies to influence the gut microbiota maturation in piglets.

Bolhuis J.E.、Kleerebezem M.、Choudhury R.、Middelkoop A.、Kemp B.、Gerrits W.J.J.

Adaptation Physiology Group, Department of Animal Sciences, Wageningen University & ResearchHost-Microbe Interactomics Group, Department of Animal Sciences, Wageningen University & ResearchHost-Microbe Interactomics Group, Department of Animal Sciences, Wageningen University & ResearchAdaptation Physiology Group, Department of Animal Sciences, Wageningen University & ResearchAdaptation Physiology Group, Department of Animal Sciences, Wageningen University & ResearchAnimal Nutrition Group, Department of Animal Sciences, Wageningen University & Research

10.1101/2020.09.30.320275

微生物学生理学动物学

early-lifedevelopmentfibrefeeding strategiesmicrobiotaneonatalpigsucklingweaning

Bolhuis J.E.,Kleerebezem M.,Choudhury R.,Middelkoop A.,Kemp B.,Gerrits W.J.J..Early-life feeding accelerates gut microbiome maturation in piglets[EB/OL].(2025-03-28)[2025-04-26].https://www.biorxiv.org/content/10.1101/2020.09.30.320275.点此复制

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