水稻滇粳优1号近等基因系的鉴定

In this study, a total of 567 SSR molecular markers distributed in the rice genome were used to identify Dianjingyou 1(DJY) and twelve near-isogenic lines (NILs), which show divergent heading date from Dianjingyou 1. By comparing the polymorphic loci, searching the introduced fragments in NILs, analyzing the known heading date genes within the fragments, the cause of the change in heading date were analyzed. As the results indicated, DJ1 and DJ2 derived from the same source, and the recessive gene dth3 from wild rice may delay their heading date; whereas Y4284 flowers earier than DJY due to the recessive allele in Hd6 loci. In addition, Y8266 and Y8296 contain the same exogenous sections, HGW and Hd1 were located in; the role of these genes needs to be further established. Besides, the background in other NILs was complicated and they need to backcross. The identification of these NILs paves the way for novel gene cloning and provides the opportunity for gene-gene interaction analysis by constructing double or triple mutants in the same background.