美国稻品种Lemont花粉育性中性基因Sc-n的鉴定

Pollen abortion is a common phenomenon in indica and japonica hybrid F1, which extremely restrict the utilization of heterosis. There are at least six gene loci, including Sa, Sb and Sc controlling pollen fertility of hybrid F1 of indica and japonica. The pollen fertility neutral genes in the six loci maybe overcome the pollen sterility of hybrid F1. Therefore, it's very important to excavate the neutral genes in each locus. In the present study, an American rice variety, Lemont, was used for pollen fertility neutral genes detection, which was crossed with Taichung 65 and its pollen sterile near-isogenic lines. The F1s with different pollen sterility loci interactions were obtained from the test cross. Firstly, the pollen fertility rate and setting rate were investigated and was found that there is no significant difference in them between Sc interacted hybrid F1 and no pollen sterility loci (Sa、Sb and Sc) interacted hybrid F1, suggesting that there was maybe pollen fertility neutral gene in Sc locus. Then SSR markers that are closer linkage with Sc locus was used for detecting F2 population and the result showed that the segregation rate of three genotype ( Sc-i Sc-i, Sc-i Sc-j, Sc-j Sc-j ) was conformed with 1:2:1. These results demonstrated that Lemont contained a neutral gene"Sc-n"in Sc locus, which may play an important role to overcome pollen semi-sterility caused by Sc interaction of indica and japonica hybrid F1 .