P7b 在耐药喉癌细胞系中表达与顺铂耐药的相关性

Objective: To define the relationship between the ATP7b expression and cisplatin resistance of laryngeal carcinoma cells. Methods: Hep-2 and Hep-2/VCR were given cisplatin for 24 h and 48 h, applied cell proliferation assays and Tunel assay to identify the differences between Hep-2 and Hep-2/VCR on cisplatin resistance. Applied immunofluorescence staining and PCR method for the changes of ATP7B in the two kinds of cells after adding cisplatin. Results: Hep-2 cell survival was 71%, after being given 50 uM and 100 uM cisplatin for 24 h, while Hep-2/VCR survived without influence. ( p<.0.01 ) ATP7A and ATP7B gene transcriptional changes were detected by real-time quantitative PCR assay after different time of being given cisplatin. It was found that 50 uM could induce Hep-2 and Hep-2/VCR to express ATP7B. ATP7B expression in Hep-2 cells enhanced at 3 h, peaked at 6 h, but down regulated at 12 h. ATP7B expression in Hep-2/VCR cells time enhanced at 3 h, peaked at 6 h, persist high expression at 24 h. Conclusion: The high expression of ATP7B related with cisplatin resistance of laryngeal squamous cell carcinoma.