猪干扰素诱导跨膜蛋白抑制JEV和EMCV的复制
Porcine Interferon-induced transmembrane proteins are inhibitors of JEV and EMCV replication
为研究猪干扰素诱导跨膜蛋白（IFITM）家族的全基因序列及其抗病毒性能，经RT-PCR和RACE技术扩增、克隆并测序，共获得3个猪IFITM家族蛋白。利用pCMV-Myc载体构建重组质粒pCMV-IFITM1/2/3，通过Western blot检测方法确认重组质粒在BHK-21细胞系中表达情况，通过real-time RT-PCR和噬斑方法检测日本乙型脑炎病毒（JEV）和脑心肌炎病毒（EMCV）在IFITM过量表达下的复制情况。结果表明：猪IFITM家族与人IFITM家族结构相似，但3'端亲水区多出13个氨基酸。猪IFITM家族的3个蛋白均可以抑制JEV和EMCV在体外易感细胞中的复制，其中以IFITM3抗病毒效果最显著。本研究结果丰富了IFITM抗病毒谱，为干扰素刺激基因在人畜共患病防治中的应用奠定了基础。
In order to study the full gene sequences and antiviral properties of porcine interferon-induced transmembrane family members, three of them were amplified using RT-PCR and RACE and cloned into the vector pCMV-Myc separately. Overexpression of these three proteins in BHK-21 cell lines were identified by Western Blot. The replication abilities of both Japanese Encephalitis Virus (JEV) and Encephalomyocarditis virus (EMCV) when IFITM is overexpressed were detected using real-time PCR and plaques. Our data reveal that the amino acid sequences of porcine IFITM family members are quite similar to that of human IFITM proteins, except for the additional 13 amino acids in the 3' terminal hydrophilic region. All of the three porcine IFITM family members inhibit the replication of JEV and EMCV in vitro, and the IFITM3 protein shows the strongest antiviral activities. These results have enriched the antiviral spectrum of IFITM proteins and laid the foundation for the application of interferon-stimulated genes in zoonosis prevention.
盖新娜、王星晨、郭鑫
基础医学分子生物学生物科学研究方法、生物科学研究技术
兽医免疫学干扰素诱导跨膜蛋白抗病毒日本乙型脑炎病毒脑心肌炎病毒
Veterinary Immunologyinterferon-induced transmembrane proteinantiviralJapanese Encephalitis VirusEncephalomyocarditis virus
盖新娜,王星晨,郭鑫.猪干扰素诱导跨膜蛋白抑制JEV和EMCV的复制[EB/OL].(2016-05-17)[2025-08-11].http://www.paper.edu.cn/releasepaper/content/201605-513.点此复制
评论