高血压对大鼠血管平滑肌细胞结缔组织生长因子表达水平的影响及其意义

IM: To explore the varied expression level of connective tissue growth factor (CTGF) in rat vascular smooth muscle cells (VSMCs) and its significance. METHODS: Using age matched Wistar-Kyoto (WKY) rats as control, the lumen diameter (L), media thickness (M), media/lumen ratio (M/L) from 4-week-old and 16-week-old SHR were detected respectively. Then the protein expression in vascular of WKY rats and SHR was detected by immunohistochemistral techniques and Western blot, and the mRNA level was analyzed by real-time reverse transcription- polymerase chain reaction with SYBR (RT-qPCR). RESULTS: The ratio of L, M and M/L in 4-week-old SHR were similar to that in 4-week-old WKY rats (P>0.05), and the protein and mRNA level of ColⅢ in vascular of 4-week-old SHR were also similar to that of age matched rats WKY rats, but CTGF expression was upregulated significantly (P<0.01). In 16-week-old SHR, the ratio of M/L were lower compared with age matched WKY rats (P<0.01). The protein and mRNA level of ColⅢ and CTGF in 16-week-old SHR were great higher than that of 16-week-old WKY (P<0.01). CONCLUSION: the abnormal hemodynamic changes might regulate CTGF expression in VSMCs. The increased expression of CTGF might contribute to vascular remodeling in hypertension.