严重烫伤大鼠肝脏库普弗细胞脂多糖受体CD14膜蛋白的表达及作用

Objective: In this paper to study lipopolysaccharide receptor CD14 membrane protein in severe burns expression in rat liver Kupffer cells, and preliminary observations the expression of the downstream proinflammatory of TNF -α and IL -1β. Method: Depend on scalded rat model, observed by immunohistochemistry and Western-blot analysis the liver Pufu cell lipopolysaccharide receptor CD14 membrane protein expression on severe burned rats in different time points, ELISA for detection of liver cell homogenate supernatant of TNF-α and IL-1β in content. Results: CD14 expression of rat liver after burn compared with the sham scald group gradually increasing trend over time, peaked at 9h, the expression began to weaken at 12h, but still significantly higher than normal levels (p <0.05). The results of ELISA show that the expression of promote inflammatory cytokines TNF - α and IL -1β have significant differences compared with fake group in scalded rat liver tissue in 12h, and to display gradually increasing trend. Conclusions: CD14 may be involved in the inflammatory response after burns, laid the foundation for further research to understand the LPS-CD14-P38MAPK shaft signaling pathway in the mechanism of the systemic inflammatory response after burn.