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不同地下滴灌制度下黄瓜根际微生物活性及功能多样性

Microbial activity and functional diversity in rhizosphere of cucumber under different subsurface drip irrigation scheduling

中文摘要英文摘要

采用微生物培养、BIOLOG碳素利用法和土壤酶活性测定等方法,分析了不同地下灌溉制度下黄瓜根际土壤中微生物活性及功能多样性。试验结果表明,根际土壤微生物C、N含量、基础呼吸、代谢熵、AWCD值、Shannon指数和McIntosh指数随灌水量的增加呈先升高后下降的趋势,两个灌水周期均是Kcp2处理最高;在Kcp2灌溉水平下,I2处理根际土壤微生物C、N含量、基础呼吸、代谢熵、AWCD值、Shannon指数和McIntosh指数显著高于I1处理。灌水周期和灌水量对Simpson指数无显著影响。Kcp2处理下,细菌、放线菌、自生固氮菌数量,脲酶、磷酸酶、蔗糖酶、过氧化氢酶和多酚氧化酶活性显著高于其它2个处理;而真菌数量随灌水量的增多而显著升高;I2处理的细菌和自生固氮菌数量、脲酶、磷酸酶和蔗糖酶活性显著高于I1处理,放线菌数量、过氧化氢酶和多酚氧化酶活性与I1处理差异不显著,而真菌数量显著低于I1处理。Kcp2I2灌水处理使得黄瓜根际土壤中微生物代谢活性和微生物群落功能多样性升高,微生物区系得以改善,土壤酶活性提高,促进黄瓜植株的生长。

In this paper, the effects of subsurface drip irrigation scheduling on microbial activity and functional diversity in rhizosphere of cucumber in solar greenhouse were studied. The results showed that the soil microbial biomass C and N, basal respiration, metabolic quotient and values of AWCD, Shannon and McIntosh indexes were increased and then decreased with improvement of irrigation water amount, and the highest values were observed in Kcp2 treatment in both irrigation intervals. The values of microbial C and N, basal respiration and metabolic quotient in I2 treatments were significantly higher than those in I1 treatments under Kcp2 irrigation level. But as for Simpson index, no significant difference was observed in all treatments. Under Kcp2 treatments, the numbers of bacteria, actinomyces and nitrogen fixing bacteria, and the activities of urease, phosphatase, sucrase, catalase and polyhenoloxidase were significantly higher than other treatments, but the fungi number was increased with improvement of irrigation water amount. The numbers of bacteria and nitrogen fixing bacteria, the activities of urease, phosphatase and sucrase in I2 treatments were significantly higher than those in I1 treatment, the actinomyces number and activities of catalase and polyhenoloxidase were no significant difference between I1 and I2 treatents, but the fungi number were significantly lower than I1 treaments under Kcp 2 irrigation level. The microbial activity and functional diversity in rhizosphere of cucumber rhizosphere were increased under Kcp2I2 treatment, meanwhile, the soil microflora was improved and the soil enzymes activities were increased, thus the cucumber growth was promoted.

李华、张自坤

农业科学研究植物学微生物学

黄瓜BIOLOG碳素利用基础呼吸微生物生物量酶活性

cucumberBIOLOGbasal respirationmicrobial biomasssoil enzymes

李华,张自坤.不同地下滴灌制度下黄瓜根际微生物活性及功能多样性[EB/OL].(2013-09-18)[2025-08-10].http://www.paper.edu.cn/releasepaper/content/201309-297.点此复制

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