吸烟致大鼠肺组织RAGE和S100A6蛋白高表达

igarette smoke has been widely investigated in terms of epidemiological and pathological observation in relation to human lung diseases. In this study, we established an animal model exposed to cigarette smoke and looked for the potential molecular mechanisms at the protein level. Wistar rats were exposed to cigarette smoke at concentrations of 20% and 60% as the low and high concentration group. The exposure was conducted twice a day、5 days a week for 43 weeks. As a major metabolite of nicotine in cigarette, cotinine in rat urine was determined by LC-MS. A time-and dose-dependent analysis indicated that the cotinine level may be used as a biomarker of smoke exposure. Expression of receptor for advanced glycation endproducts(RAGE), an immunoglobulin superfamily that triggers the intracellular signal cascade reaction leading to inflammation and its ligand S100A6(calgranulin) in bronchial epithelial cells and lung tissues of rats exposed to cigarette smoke, were found to be correlated with the cotinine level, indicating that RAGE and S100A6 may attribute to the inflammation and oxidative damage caused by cigarette smoke.