自噬在叶锈菌侵染小麦过程中的作用

In this paper，we systematically investigated the formation characteristics of autophagy in leaf rust fungus and the function of autophagy in process of leaf rust infecting the wheat. By using autophagy specific marker MDC staining and confocal observation to detect whether the leaf rust fungus infection structures have autophagy, combining with electron microscopy observation to conform the characteristics of autophagy information. The affection of autophagy to leaf rust fungus growth was observed following the injection of 3-MA(an inhibitor of autophagy synthesis). The results showed in the different structures of leaf rust fungus formation, autophagy could be observed rarely in germ tubes, appressoriums, sub-stomatal vesicles, intercellular hyphas and haustorium except haustorial mother cell. The expanding area of intercellular hyphas and the ability of spores formation were deeply inhibited by 3-MA. So we believed that the autophagy in haustorial mother cell played an important role in leaf rust fungus infection.