苦马豆素对小鼠细胞免疫功能的影响

Objective：The purpose was to study the relationship of cellular immune function with different doses of SW in mice. All these can provide new thoughtful method for the study of SW on immuno-toxicology. Method：Eighty mice were divided into 8 groups randomly. Proliferative responses of spleen lymphocyte, immune organs index, cellular numbers were detected with 0.05、0.2、0.8、3.2 and 6.4 mg/kg SW by intragastric administration for 21 days. Three other groups were physiologic saline, cyclophosphamide and normal groups. Proliferative response of normal spleen lymphocyte were detected with SW doses of 0.002、0.02、0.2、2 and 4 μg/mL. Result：The stimulation index value (SI) of spleen lymphocyte with unstimulated and stimulated to ConA was increased with low doses of SW and decrease with high doses of SW. The tendency was the same as thymus index. Proliferative responses of lymphocyte stimulated to PHA-P were decreased with dose dependent of SW, but not sensitive to LPS. Cellular numbers were decreased. Conclusion：SW depressed cellular immune function by intragastric administration for 21 days at the dose over 6.4 mg/kg；SW depressed the proliferative response of spleen lymphocyte directly at the dose over 0.02 μg/mL. SW improved the function of lymphocyte to some degree at low dose of SW. These results suggest that SW can influence immuno-function by selectivity and two-way regulation with suitable doses of SW.