黄芪和三七的四种有效成分配伍对脑缺血再灌注后小鼠脑组织氧化应激的影响

Objective: To investigate the influence of astragaloside Ⅳ (the effective component of Astragalus) combined with ginsenoside Rg1, ginsenoside Rb1, notoginsenoside R1 (the effective components of Panax notoginseng) on oxidative stress after cerebral ischemic-referfusion in mice. Method: C56BL/6 mice were randomly divided into eleven group, then, models of cerebral ischemia-reperfusion injury were prepared by bilateral common carotid artery ligation for 20 min followed by 24 h reperfusion after treated for 3 days. Brain tissue was taken to yield a 10% homogenate, to detect the contents of Malonaldehyde (MDA), Nitric Oxide (NO), Glutathione (GSH) and the activity of Superoxide Dismutase (SOD) in brain tissue after reperfusion. Result: 1. Compared with the sham group, the contents of MDA、NO significantly were increased (all P<0.01), SOD activity and GSH level were decreased in the model group (P<0.01 or P<0.05). Compared with the model group, astragaloside Ⅳ significantly inhibited the increase in MDA, NO (all P<0.01), was less influenced on SOD, GSH (P>0.05); ginsenoside Rg1 reduced obviously NO content, had no marked effects on MDA, SOD, GSH (P>0.05); No significant effects (P>0.05) were all found on MDA, NO, SOD, GSH in ginsenoside Rb1 and notoginsenoside R1. 2. Astragaloside Ⅳ combined respectively with the active components of Panax notoginseng (two active components combination) all reduced obviously the contents of MDA and NO (P<0.01or P<0.05), and, the effects in astragaloside Ⅳ+ginsenoside Rb1 and astragaloside Ⅳ+notoginsenoside R1 were better than those in ginsenoside Rb1, notoginsenoside R1 alone; however, two active components combination had all no effects on SOD activity and GSH level (P>0.05). 3. Four active components combination decreased significantly the contents of MDA and NO, increased significantly SOD activity and GSH level (all P<0.01), furthermore, the effects were more obvious than those in the single-drug and two active components combination (P<0.01 or P<0.05). 4. Astragaloside Ⅳ, two or four active components combination decreased MDA, which did not been find in the active components of Panax notoginseng alone. Astragaloside Ⅳ, ginsenoside Rg1, two or four active components combination could inhibit the produce of NO. Four active component alone, two active components combination had no marked effects on SOD and GSH, but which were increased obviously in four active components combination. Conclusion: Combination between Astragaloside Ⅳ (40 mg.kg-1), ginsenoside Rg1 (50 mg.kg-1), ginsenoside Rb1 (40 mg.kg-1) and notoginsenoside R1 (10 mg.kg-1) inhibited significantly the early oxidative stress injury after cerebral ischemia-reperfusion in mice, promoted the protective effect on brain tissue. And the effect on MDA was mainly Astragaloside Ⅳ, the effect on NO was mainly astragaloside Ⅳ and ginsenoside Rg1, the effects on SOD activity and GSH level were four active components combination.