Foxp3基因转染间充质干细胞的免疫特性

In this paper, we sought to determine whether or not MSCs transfected with Foxp3 (MSC-Foxp3) exhibited stronger immunosuppressive activity than MSC-expressing vector (MSC-Mock) alone, and, if yes, define the essential mechanisms for this to occur. Successful gene transfer of Foxp3 was confirmed by Western blot analyses and Foxp3-engineered MSCs possess both the phenotype and differentiation ability associated with MSCs. Foxp3 transduction significantly improved the immunosuppressive activity of MSCs and involved both soluble mediators and cell contact-dependent mechanisms. Foxp3 transduction significantly increased the distribution of cell surface molecule neuropilin-1(Nrp-1) and costimulatory molecule programmed death ligand 1(PD-L1) in MSCs. It also promoted the induction of CD4+CD25+Foxp3+ Tregs and the production of soluble immunomodulatory factors interleukin-10 (IL-10) and transforming growth factor β (TGF-β). The findings suggest that Foxp3-engineered MSC therapy could be a promising and attractive cell therapy approach for inducing immunosuppression or transplant tolerance.an)