人参皂苷Rd口服吸收及其体内药代动力学的研究

im To develop a HPLC method for the determination of ginsenoside Rd in mice plasma ,and to study the oral bioavailability of ginsenoside Rd and the time course of Rd contents in the digestive tract after oral administration, and to investigate the characteristics of pharmacokinetic behavior of ginsenoside Rd in the treatment of ulcerative colitis. Methods After intravenous administration and oral administration, the concentrations of drug in mice blood were recorded; such samples as duodenum ,jejunum, ileum and colon included their contents at different time points after oral administration were taken out and analyzed by HPLC. Results It showed that Rd in plasma were not detected after oral administration. The amount of Rd in the small intestine 1 hour after administration were 24.0±2.3% of the dose. Two hours later ,when most of the Rd in the jejunum transferred into the ileum ,the amount of Rd in the jejunum decreased to only 5.0±1.0% of the dose and that in the ileum increased largely to 23.0±6.0% of the dose. Then, that in the colon 5 hours later was as high as 8.0±4.4% of the dose, 6 hours later ,that in the small intestine was near the limit of detection and , on the contrary , 19.8±13.7% of the dose was found in the colon. Ten hours later ,ginsenoside Rd was not detected in the colon. Conclusion The method was sensitive, accurate, rapid and can be applied to determine the ginsenoside Rd in plasma. Most of the Rd was found in the colon after oral administration, it is the pharmcokinetic basis of ginsenoside Rd in the treatment of ulcerative colitis.