N基因导入对自发性高血压大鼠肾脏纤维化的影响
he effects of human decorin gene on renal fibrosis in spontaneously hypertensive rats
目的 研究人核心蛋白聚糖（DCN）基因导入对自发性高血压大鼠(spontaneously hypertensive rats, SHR)肾脏纤维化的逆转作用。方法 16周龄雄性SHR经尾静脉注入重组腺相关病毒(recombinant adeno-associated virus, rAAV)介导的人DCN病毒。16周后处死动物测定人DCN基因在各脏器中的表达及肾脏纤维化程度。结果 经尾静脉注入重组腺相关病毒介导的人DCN基因后，高血压大鼠的肾脏纤维化程度较对照组明显改善。结论 人DCN基因的导入可预防和逆转高血压所致的肾脏纤维化，为临床应用DCN基因治疗合并有肾脏损伤的高血压提供了可能性。
Objective To investigate the reversal effects of DCN gene on SHR's renal fibrosis. Methods 16 week male SHRs were allocated randomly into three groups: saline (1ml), a single dose of rAAV-GFP or rAAV-DCN (about 1×1011 virion particles per rat in 1 ml of saline solution) via the tail vein into SHRs were performed respectively. At the end, we measured rats' renal fibrosis. The expression of DCN in the heart, kidney and liver were detected by RT-PCR. Results After delivery of human DCN in SHRs, renal fibrosis has been obviously improved (P<0. 05). Conclusion Human DCN gene can reverse renal fibrosis in SHRs resulting from hypertension.
严雯、汪道文、冯敏
基础医学内科学
高血压肾脏纤维化人核心蛋白聚糖基因
hypertensionrenal fibrosisdecorin
严雯,汪道文,冯敏.N基因导入对自发性高血压大鼠肾脏纤维化的影响[EB/OL].(2013-09-17)[2025-08-16].http://www.paper.edu.cn/releasepaper/content/201309-244.点此复制
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