古树大理茶优势内生真菌 1 个新四氢-β-咔啉二酮哌嗪的分离鉴定
为挖掘古树大理茶优势内生真菌间座壳属菌株 Diaporthe tectonicgena 的化学成 分，采用硅胶、大孔吸附树脂 Diaion HP20、葡聚糖凝胶 LH-20 等柱层析方法，对该菌株 的大米固态发酵提取物进行分离纯化，并通过 HRMS H NMR 13C NMR、HSQC、HMBC 和 COSY等波谱分析，对所得化合物进行结构鉴定。结果表明：（1）从该菌株大米固态发酵 提取物中分离得到 4 个化合物，其中新化合物 1 鉴定为四氢-β-咔啉二酮哌嗪类生物碱，命 名为tectonicgenazine A。（2）3 个已知化合物分别鉴定为 trans-cyclo-(D-tryptophanyl-Ltyrosyl)（ 2）、1H-吲哚-3-羧酸-2,3-二羟基丙酯（3）和 N-羟乙基-2-乙酰基吡咯（4），其中 化合物3 为首次从自然界中分离所得。
o investigate the chemical constituents of Diaporthe tectonicgena, a predominant endophytic fungus in the ancient tea tree of Camellia taliensis, the rice solid-state fermentation extract was isolated and purified by silica gel, Diaion HP20 and Sephadex LH-20 column chromatographies, and the chemical structures were elucidated by extensive 1H NMR, 13C NMR, HSQC, HMBC, COSY and HR-MS spectroscopic analyses. The results were as follows: (1) Four compounds are isolated from the rice solid-state fermentation extract of Diaporthe tectonicgena, including a new tetrahydro--carboline diketopiperazine alkaloid named tectonicgenazine A (1); (2) Three known compounds, trans-cyclo-(D-tryptophanyl-L-tyrosyl) (2), 1H-indole-3-carboxylic acid-2,3-dihydroxypropyl ester (3) and N-hydroxyethyl-2-acetylpyrrole (4) were obtained and identified, and compound 3 was isolated from nature for the first time.
朱国磊、黄永林、陈肖学、程璐瑶、赵 平、李 娜、张颖君
生物科学研究方法、生物科学研究技术生物化学微生物学
大理茶，内生真菌，间座壳属，固态发酵，二酮哌嗪
朱国磊,黄永林,陈肖学,程璐瑶,赵 平,李 娜,张颖君.古树大理茶优势内生真菌 1 个新四氢-β-咔啉二酮哌嗪的分离鉴定[EB/OL].(2022-10-27)[2025-08-16].https://chinaxiv.org/abs/202211.00041.点此复制
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