Electric Egg-Laying: Effect of Electric Field in a Microchannel on C. elegans Egg-Laying Behavior
Electric Egg-Laying: Effect of Electric Field in a Microchannel on C. elegans Egg-Laying Behavior
ABSTRACT In this paper, the novel effect of electric field (EF) on adult C. elegans egg-laying in a microchannel is discovered and correlated with neural and muscular activities. The quantitative effects of worm aging and EF strength, direction, and exposure duration on egg-laying is studied phenotypically using egg-count, body length, head movement, and transient neuronal activity readouts. Electric egg-laying rate increases significantly when worms face the anode and the response is EF-dependent, i.e. stronger (6V/cm) and longer EF (40s) exposure result in a shorter egg laying response duration. Worm aging significantly deteriorates the electric egg-laying behaviour with 88% decrease in the egg-count from Day-1 to Day-4 post young-adult stage. Fluorescent imaging of intracellular calcium dynamics in the main parts of the egg-laying neural circuit demonstrate the involvement and sensitivity of the serotonergic hermaphrodite specific neurons (HSNs), vulva muscles, and ventral cord neurons to the EF. HSN mutation also results in a reduced rate of electric egg-laying allowing the use of this technique for cellular screening and mapping of the neural basis of electrosensation in C. elegans. This novel assay can be parallelized and performed in a high-throughput manner for drug and gene screening applications.
Rezai Pouya、Archonta Daphne、Youssef Khaled、Tandon Anurag、Kubiseski Terrance J.
Department of Mechanical Engineering, York UniversityDepartment of Mechanical Engineering, York UniversityDepartment of Mechanical Engineering, York UniversityTanz Centre for Research in Neurodegenerative Diseases||Department of Medicine, University of TorontoDepartment of Biology, York University
生物科学现状、生物科学发展生物科学研究方法、生物科学研究技术生物物理学
MicrofluidicsC. elegansElectric fieldEgg-laying
Rezai Pouya,Archonta Daphne,Youssef Khaled,Tandon Anurag,Kubiseski Terrance J..Electric Egg-Laying: Effect of Electric Field in a Microchannel on C. elegans Egg-Laying Behavior[EB/OL].(2025-03-28)[2025-05-18].https://www.biorxiv.org/content/10.1101/2020.09.01.276097.点此复制
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